Isolation and phenotypic identification of some moulds isolated from Potato chips sold in Birnin Kebbi, Nigeria

Abstract

Potato chips are widely consumed ready-to-eat snacks and are prone to fungal contamination during processing, packaging, storage, and marketing. This study investigated the isolation and phenotypic identification of molds associated with potato chips sold in Birnin Kebbi, Nigeria. A total of fifty (50) potato chip samples were purchased from major markets and street vendors within Birnin Kebbi metropolis. Samples were analyzed using standard mycological techniques. Isolation was carried out on Sabouraud Dextrose Agar supplemented with chloramphenicol. Identification of isolates was based on macroscopic colony characteristics and microscopic morphology using lactophenol cotton blue staining. Results showed that 80% of the samples were contaminated with molds, while 20% showed no fungal growth. The predominant molds isolated belonged to the genus Aspergillus, with Aspergillus niger (60%) and Aspergillus flavus (40%) being identified. The presence of these molds, known for their spoilage potential and ability to produce mycotoxins, suggests possible public health risks associated with the consumption of contaminated potato chips. Improved hygienic practices during processing, storage, and marketing are therefore recommended.