ANTIBACTERIAL SUSCEPTIBILITY PATTERN OF BACTERIA ISOLATED FROM URINE SAMPLES OFPATIENTS WITH URINARY TRACT INFECTIONS ATTENDING GENERAL HOSPITAL MINNA, NIGER STATE, NIGERIA

Abstract

This study determined the antibacterial susceptibility pattern of bacterial isolates from urine samples of patients attending General Hospital, Minna, Niger State. Four hundred (400) urine samples were collected and cultured on Cysteine Lactose Electrolyte Deficient agar (CLED), MacConkey and Nutrient agar for isolation of bacteria. The isolated bacteria were identified using colonial, microscopic and biochemical tests. Antibacterial susceptibility profiles of the isolates were carried out using disc diffusion method.The bacteria isolates identified include: Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus luteus, Salmonella enterica, Streptococcus pneumoniae, Enterobacter faecalis and Serratia marcescens. Escherichiacoli 25(30.1 %) occurred more frequency than other bacterial isolates, followed by Klebsiella pneumoniae 20(24.0 %) and Staphylococcus aureus 16(19.3 %) Enterobacter faecalis 2(2.4 %) and Serratia marcescens 2(2.4 %) recorded theleast bacterial isolates. Female patients recorded more bacterial isolates than their male counterparts. Age groups 15-24 years revealed diverse bacteria, when compared to other age groups. Married patients recorded higher prevalence rate of bacterial infections 46(55.4 %), when compared to patients that were single 37(44.5 %). Similarly, patients with no formal education 35(42.1 %) were infected with diverse bacteria, when compared to those with formal education 11(13.2 %). Salmonella enterica 2(66.7 %) recorded the highest susceptibility to Nalidixic acid, Gentamicin and Sulfamethoxazole-trimepthoprim while, Staphylococcus aureus 16(62.5 %) and Streptococcuspneumoniae, 3(66.7 %) were susceptible to Levofloxacin, Chloramphenicol and Amoxil. Similarly, all the isolated bacteria were resistant to atleast three (3) antibiotic tested (MDRI=0.3). Multidrug resistance uropathogens exist in this study area. Therefore, regular surveillance and stewardship should be encouraged.