http://irepo.futminna.edu.ng:8080/jspui/handle/123456789/114 2026-05-05T08:49:45Z 2026-05-05T08:49:45Z Oyewole, O.A., Binuyo, O.A., Joda, J.F., Nabara, H.Y., Tsado, P.Y., Musa, F., Akinpelu, O.J. http://irepo.futminna.edu.ng:8080/jspui/handle/123456789/30549 2026-04-19T16:27:10Z 2024-01-01T00:00:00Z

Title: Isolation and screening of xylanase-producing microorganisms. Authors: Oyewole, O.A.,; Binuyo, O.A.,; Joda, J.F.,; Nabara, H.Y.,; Tsado, P.Y.,; Musa, F.,; Akinpelu, O.J. Abstract: Xylanases are enzymes that break down the complex polysaccharide xylan, the major component of hemicellulose. Xylanase hydrolyzes xylan by breaking the β-1,4- glycoside linkage to produce xylose and other degradation compounds. Many degradative microorganisms such as fungi, bacteria and yeast have been found to produce xylanases. In this study, xylanase-producing microorganisms were isolated from different environmental samples - paddy field rhizosphere, earthen pond sludge, decayed wood, cow dung, and sediments from Tagwai Dam, Minna, Nigeria. The samples were serially diluted and plated on a selective agar medium, which contains xylan (1.5 g), peptone (5 g), yeast extract (3 g), sodium chloride (5 g), and agar (15 g) per 1 L of distilled water using spread plate method and incubated at 37 °C for 48 hours and 25 °C for 5 days for bacteria and fungi respectively. The plate screening method was used to screen the microbial isolates for xylanase activity. Colonies with zones of inhibition indicated xylanase activity. Positive isolates were further subcultured to obtain pure isolates and were kept in slant bottles and stored at 4°C for further analysis. The pure isolates were cultured in nutrient broth for bacteria and Sabouraud dextrose broth for fungi and from the broth, into selective broth which was used to determine xylanase activity using DNS (dinitrosalicylic acid) method. The bacterial isolates were identified based on their cultural, morphological and biochemical characteristics while the fungal isolates were stained with lactophenol cotton blue. Ten (10) bacteria were isolated from the samples, of which four (Clostridium sp., Escherichia coli, Salmonella sp., and Staphylococcus sp) were xylanase producers and four (4) xylanase-producing fungi (Aspergillus niger, A. fumigatus, A. flavus, and A. sydowii were identified for fungi) were isolated. Escherichia coli gave the highest activity for the bacteria (0.8260) while Aspergillus flavus gave the highest xylanase activity for fungi (2.1118). Aspergillus flavus can therefore be recommended for large-scale production of xylanase.

2024-01-01T00:00:00Z Gana, M., Abioye, O.P., Oyewole, O.A., Akande, K.O., Haruna, A. http://irepo.futminna.edu.ng:8080/jspui/handle/123456789/30548 2026-04-19T01:18:38Z 2026-01-01T00:00:00Z

Title: Microbial production of lysine from agrowastes. Authors: Gana, M.,; Abioye, O.P.,; Oyewole, O.A.,; Akande, K.O.,; Haruna, A. Abstract: Lysine is a crucial amino acid that the body cannot produce but critical for overall health. Conventional lysine production is expensive, hazardous to the environment, and depends on refined substrates; therefore, a sustainable alternative that uses agricultural waste is required. The aim of this study was to produce lysine by bacteria using agro wastes as substrate. The objectives include isolating and screening lysine producing bacteria from soil sample, characterizing and identifying the lysine producing bacteria, screening agro-wastes (groundnut shell, corn cob, and sugarcane bagasse), optimizing the conditions for production and extracting and purifying lysine. Bacillus subtilis was isolated and utilized for fermentation while Gas Chromatography-Mass Spectrometry (GC-MS) was employed for lysine quantification. The result showed that the three agro wastes were found to support lysine production, serving as carbon sources. However, corn cob poses to be the best carbon source for the bacterium. B. subtilis with lysine concentration of 1.41g/L under pH of 7, temperature of 32.50 °C, and a carbon concentration of 12.50% . The genetic modification of Bacillus subtilis to enhance its metabolic pathways for lysine synthesis should be the primary focus of further research and development.

2026-01-01T00:00:00Z Nathaniel, V., Ijah, U.J.J., Oyewole, O.A., Anuonye, J.C., Abdulmalik, S.U http://irepo.futminna.edu.ng:8080/jspui/handle/123456789/30395 2026-03-24T00:59:32Z 2026-01-01T00:00:00Z

Title: Bacteriocin Production by Lactococcus garvieae MF098136.1 Isolated from Locally Fermented Foods for Food Preservation Authors: Nathaniel, V.,; Ijah, U.J.J.,; Oyewole, O.A.,; Anuonye, J.C.,; Abdulmalik, S.U Abstract: Bacteriocins are natural antimicrobial peptides primarily produced by lactic acid bacteria (LAB). This study focused on the production of bacteriocins from LAB isolated from locally fermented Nigerian foods. Isolation of LAB was performed on anaerobic de Man, Rogosa, and Sharpe agar (MRS) agar at 37°C for 72 hours, followed by morphological, biochemical and molecular characterization. The bacteriocins were produced from the LAB, optimised, purified and tested against food spoilage bacteria. The proximate composition was determined and the toxicological examination of the bacteriocin was evaluated in albino rats. A total of 50 LAB isolates were obtained. The LAB with the best potential was identified as Lactococcus garvieae MF098136.1 and exhibited significant antibacterial activity against pathogens isolated from the food samples. The optimal bacteriocin production by L. garvieae MF098136.1 reached maximum activity at pH 5.28, 72 hours incubation, and 20°C. Purification processes enhanced bacteriocin specific activity up to 12.7 AU/mg but reduced overall yield (23–55%). Toxicological evaluation in albino rats at doses up to 5000 mg/kg revealed no mortality or significant adverse effects. The bacteriocin significantly suppressed microbial load in stored Akamu and Masa, compared to controls. Additionally, the bacteriocin treatments enhanced sensory properties and overall acceptability.

2026-01-01T00:00:00Z Oyewole, O.A., Jagaba, A. Abdulhammed, A. A. Yakubu, J. G., Mohammed, M. A., Abioye, O.P., Adeniyi, D. O., http://irepo.futminna.edu.ng:8080/jspui/handle/123456789/30378 2026-03-17T22:10:00Z 2023-01-01T00:00:00Z

Title: Investigating the Bioflocculant Production Potential of Microorganisms Isolated From Earthen Pond Sludge Authors: Oyewole, O.A.,; Jagaba, A.; Abdulhammed, A. A.; Yakubu, J. G.,; Mohammed, M. A.,; Abioye, O.P.,; Adeniyi, D. O., Abstract: Background: Bioflocculants are biodegradable polymers produced by microorganisms and have gained applications in aggregating dissolved and suspended substances in water. This study aimed to screen, isolate and identify microorganisms from earthen pond sludge for flocculant production. Materials and Methods: Samples were collected from earthen pond sludge in Lapai Gwari in Bosso Local Government Area of Minna, Nigeria located at longitude 6.5052°E and latitude 9.5246°N, and screened for microorganisms with bioflocculating ability using kaolin suspension and selective medium containing (per liter) NaCl 0.1 g, MgSO47H2O 0.2 g, K2HPO4 5 g, agar 5.5 g, yeast extract 0.5 g, urea 0.5 g, KH2PO4 2 g, and glucose 10 g for bacteria, and MgSO47H2O 0.2 g, K2HPO4 5 g, agar 5.5 g, yeast extract 0.5 g, urea 0.5 g, KH2PO4 2 g, glucose 10 g for fungi. The lyophilized bioflocculant produced by the bacterium was characterized using thermogravimetric property analysis (TGA), Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) Results and Conclusion: Of the twelve (12) microorganisms isolated, Prestia megaterium (accession number ON184360) gave the highest potential (30 %) for flocculant production. Tyrosine-protein kinase gene was detected to be responsible for the bioflocculant production. A total of 10.67 g of bioflocculant was produced from 500 mL of the medium. The TGA, FTIR spectra and scanning electron micrograph exhibited typical characteristics of flocculant. The findings of this investigation indicated that Priestia megaterium isolated from earthen pond sludge is a suitable substitute for flocculant production.

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